Journal: Oxidative Medicine and Cellular Longevity

Article Title: A Novel Ruthenium-Fluvastatin Complex Downregulates SNCG Expression to Modulate Breast Carcinoma Cell Proliferation and Apoptosis via Activating the PI3K/Akt/mTOR/VEGF/MMP9 Pathway

doi: 10.1155/2021/5537737

Figure Lengend Snippet: The immunohistochemical analysis of the (a) p53, (b) Bcl2, (c) Bax, and (d) MMP9 expressions in the mammary tissues of different groups of rats: (A) the normal control, (B) carcinogen control, (C) 25 mg/kg complex-treated rats, (D and E) 50 and 100 mg/kg complex-treated rats, (F) 50 mg/kg fluvastatin-treated animals, and (G) 50 mg/kg ruthenium-treated animals. All images at 40x.

Article Snippet: Rabbit anti-rat p53, Bax, Bcl2, Ki-67, MMP9, and goat anti-rabbit IgG secondary antibodies were bought from AnaSpec Inc. (San Jose, CA, USA).

Techniques: Immunohistochemical staining, Control

Journal: Oxidative Medicine and Cellular Longevity

Article Title: A Novel Ruthenium-Fluvastatin Complex Downregulates SNCG Expression to Modulate Breast Carcinoma Cell Proliferation and Apoptosis via Activating the PI3K/Akt/mTOR/VEGF/MMP9 Pathway

doi: 10.1155/2021/5537737

Figure Lengend Snippet: Effect of ruthenium, fluvastatin, and the ruthenium-fluvastatin complex on the expression of Bax, Bcl2, p53, and MMP9 in breast tissues.

Article Snippet: Rabbit anti-rat p53, Bax, Bcl2, Ki-67, MMP9, and goat anti-rabbit IgG secondary antibodies were bought from AnaSpec Inc. (San Jose, CA, USA).

Techniques: Expressing, Control

Journal: International Journal of Molecular Sciences

Article Title: The Role of Hydrogen Sulfide in the Localization and Expression of p53 and Cell Death in the Nervous Tissue in Traumatic Brain Injury and Axotomy

doi: 10.3390/ijms242115708

Figure Lengend Snippet: Immunofluorescence microscopy: ( a ) expression of p53 (green fluorescence) in the cerebral cortex of the control group, which was injected with saline, and experimental groups of animals that were injected with Na 2 S and AOAA, 24 h and 7 days after TBI. Scale bar 20 µm. ( b ) Expression of p53 (green fluorescence) in the cerebral cortex of the control group, which was injected with saline, and experimental groups of animals that were injected with Na 2 S and AOAA, 7 days after TBI. Scale bar 25 µm. ( c ) p53 expression (green fluorescence) in a brain section from an animal injected with AOAA, 7 days after TBI. The white square is the area for analysis of p53 expression. White arrow is the location of the injury. Scale bar 500 µm. ( d ) Coefficient M1 of colocalization of p53 and neuronal nuclear marker NeuN in the contralateral and ipsilateral cortex of the control and experimental groups 24 h and 7 days after TBI. ( e ) Dependence of the average intensity of p53 fluorescence in neurons of the contralateral and ipsilateral cortex of the control and experimental groups 24 h and 7 days after TBI. Contra, contralateral cortex; Ipsi, ipsilateral cortex. NeuN, marker of neuron nuclei (red fluorescence); NeuN+p53 and Hoechst+p53, overlap. Hoechst—Hoechst 33342 fluorescence (blue fluorescence), which visualizes the nuclei of all cells, neurons and glia. One-way ANOVA. M ± SEM. n = 6. ** p < 0.01, *** p < 0.001—ipsilateral cortex relative to contralateral cortex of one animal; # p < 0.05, ## p < 0.01—ipsilateral cortex of the experimental group versus ipsilateral cortex of the control group within one time period after injury.

Article Snippet: Sections are then incubated with rabbit primary anti-p53 antibody (1:100; PAA928Mu01, Cloud-Clone Corp, Wuhan, China) and mouse anti-neuN protein antibody (1:1000; FNab10266, FineTest, Wuhan, China) or astrocyte marker GFAP (1:1000; SAB4200571, Sigma Aldrich) for two days at 4 °C.

Techniques: Immunofluorescence, Microscopy, Expressing, Fluorescence, Injection, Saline, Marker

Journal: International Journal of Molecular Sciences

Article Title: The Role of Hydrogen Sulfide in the Localization and Expression of p53 and Cell Death in the Nervous Tissue in Traumatic Brain Injury and Axotomy

doi: 10.3390/ijms242115708

Figure Lengend Snippet: Immunofluorescence microscopy: ( a ) expression of p53 (green fluorescence) in the cerebral cortex of the control group, which was injected with saline, and experimental groups of animals that were injected with Na 2 S and AOAA, 24 h and 7 days after TBI. Scale bar 20 µm. ( b ) Expression of p53 (green fluorescence) in the cerebral cortex of the control group, which was injected with saline, and experimental groups of animals that were injected with Na 2 S and AOAA, 7 days after TBI. Scale bar 25 µm. ( c ) p53 expression (green fluorescence) in a brain section from an animal injected with AOAA, 7 days after TBI. The white square is the area for analysis of p53 expression. White arrow is the location of the injury. Scale bar 500 µm. ( d ) Coefficient M1 of p53 colocalization and GFAP neuronal nuclear marker in the contralateral and ipsilateral cortex of the control and experimental groups 24 h and 7 days after TBI. Scale bar 20 µm. Contra, contralateral cortex; Ipsi, ipsilateral cortex. GFAP+p53 and Hoechst+p53—overlap. Hoechst—Hoechst 33342 (blue fluorescence) fluorescence, which visualizes the nuclei of all cells, neurons and glia. One-way ANOVA. M ± SEM. n = 6. * p < 0.05, ** p < 0.01, *** p < 0.001—ipsilateral cortex relative to contralateral cortex of one animal; # p < 0.05, ## p < 0.01—ipsilateral cortex of the experimental group versus ipsilateral cortex of the control group within the same time period after injury.

Article Snippet: Sections are then incubated with rabbit primary anti-p53 antibody (1:100; PAA928Mu01, Cloud-Clone Corp, Wuhan, China) and mouse anti-neuN protein antibody (1:1000; FNab10266, FineTest, Wuhan, China) or astrocyte marker GFAP (1:1000; SAB4200571, Sigma Aldrich) for two days at 4 °C.

Techniques: Immunofluorescence, Microscopy, Expressing, Fluorescence, Injection, Saline, Marker

Journal: International Journal of Molecular Sciences

Article Title: The Role of Hydrogen Sulfide in the Localization and Expression of p53 and Cell Death in the Nervous Tissue in Traumatic Brain Injury and Axotomy

doi: 10.3390/ijms242115708

Figure Lengend Snippet: Western blot analysis. Effect of Na 2 S and AOAA on p53 protein expression in the contralateral and ipsilateral cortex 24 h and 7 days after TBI. One-way ANOVA. M ± SEM. n = 6. * p < 0.05, ** p < 0.01—ipsilateral cortex relative to contralateral cortex of one animal; # p < 0.05, ## p < 0.01—ipsilateral cortex of the experimental group versus ipsilateral cortex of the control group within one time period after injury.

Article Snippet: Sections are then incubated with rabbit primary anti-p53 antibody (1:100; PAA928Mu01, Cloud-Clone Corp, Wuhan, China) and mouse anti-neuN protein antibody (1:1000; FNab10266, FineTest, Wuhan, China) or astrocyte marker GFAP (1:1000; SAB4200571, Sigma Aldrich) for two days at 4 °C.

Techniques: Western Blot, Expressing

Journal: International Journal of Molecular Sciences

Article Title: The Role of Hydrogen Sulfide in the Localization and Expression of p53 and Cell Death in the Nervous Tissue in Traumatic Brain Injury and Axotomy

doi: 10.3390/ijms242115708

Figure Lengend Snippet: Fluorescence microscopy. ( a ) Crayfish stretch receptor 6 h after axotomy, stained with Hoechst and propidium iodide, control and experimental groups, which were incubated with Na 2 S and AOAA. White dotted square—MRN; N—neuron nucleus; the white arrow—glial cells. ( b ) MRN axon section 6 h after axotomy, stained with Hoechst and propidium iodide of the control and experimental groups. White star—glial cell apoptosis. ( c ) Necrosis of glial cells surrounding the neuron body, 6 h after axotomy. ( d ) Apoptosis of glial cells MRN in a 2 mm area along the axon, was assessed by the number of fragmented glial cells. Scale bar 40 µm. Hoechst+p53—overlap. Hoechst—Hoechst 33342 (blue fluorescence) fluorescence, which visualizes the nuclei of all cells, neurons and glia. One-way ANOVA. M ± SEM. n = 6. * p < 0.05.

Article Snippet: Sections are then incubated with rabbit primary anti-p53 antibody (1:100; PAA928Mu01, Cloud-Clone Corp, Wuhan, China) and mouse anti-neuN protein antibody (1:1000; FNab10266, FineTest, Wuhan, China) or astrocyte marker GFAP (1:1000; SAB4200571, Sigma Aldrich) for two days at 4 °C.

Techniques: Fluorescence, Microscopy, Staining, Incubation

Journal: International Journal of Molecular Sciences

Article Title: The Role of Hydrogen Sulfide in the Localization and Expression of p53 and Cell Death in the Nervous Tissue in Traumatic Brain Injury and Axotomy

doi: 10.3390/ijms242115708

Figure Lengend Snippet: Immunofluorescence microscopy. ( a ) Expression of p53 (green fluorescence) in the stretch receptor of crayfish of the control group and experimental groups that were incubated with Na 2 S and AOAA, 6 after axotomy. ( b ) p53 expression (green fluorescence) in the distal axon region of the MRN of the control group and experimental groups that were incubated with Na 2 S and AOAA, 6 after axotomy. ( c ) p53 fluorescence intensity in the nucleus and cytoplasm of MRN, as well as in the nucleoplasm of glial cells 6 h after axotomy. ( d ) p53 fluorescence intensity in the proximal and distal portion of the axon, as well as in the dendritic region of the MRN. ( e ) Expression of p53 in the stretch receptor of crayfish and distal portion of the axon. ( f ) Crayfish stretch receptor in transmitted light. ( g ) Schematic of the crayfish stretch receptor. RM—receptor muscle; N—neuron nucleus; C—cytoplasm; D—dendrites; A—axon; G and white stars—glial cells; red arrows—p53 cord inside the axon; Hoechst+p53—overlap. Hoechst-Hoechst 33342 fluorescence, which visualizes the nuclei of all cells, neurons and glia. Scale bar 40 µm. One-way ANOVA. M ± SEM. n = 6. * p < 0.05, ** p < 0.01, *** p < 0.01.

Article Snippet: Sections are then incubated with rabbit primary anti-p53 antibody (1:100; PAA928Mu01, Cloud-Clone Corp, Wuhan, China) and mouse anti-neuN protein antibody (1:1000; FNab10266, FineTest, Wuhan, China) or astrocyte marker GFAP (1:1000; SAB4200571, Sigma Aldrich) for two days at 4 °C.

Techniques: Immunofluorescence, Microscopy, Expressing, Fluorescence, Incubation

Journal: International Journal of Molecular Sciences

Article Title: The Role of Hydrogen Sulfide in the Localization and Expression of p53 and Cell Death in the Nervous Tissue in Traumatic Brain Injury and Axotomy

doi: 10.3390/ijms242115708

Figure Lengend Snippet: Western blot analysis. Effect of Na 2 S and AOAA on p53 protein expression in axotomized ganglia of the ventral nerve cord 6 h after axotomy. One-way ANOVA. M ± SEM. n = 6. * p < 0.05, ** p < 0.01.

Article Snippet: Sections are then incubated with rabbit primary anti-p53 antibody (1:100; PAA928Mu01, Cloud-Clone Corp, Wuhan, China) and mouse anti-neuN protein antibody (1:1000; FNab10266, FineTest, Wuhan, China) or astrocyte marker GFAP (1:1000; SAB4200571, Sigma Aldrich) for two days at 4 °C.

Techniques: Western Blot, Expressing

Journal: International Journal of Molecular Sciences

Article Title: The Role of Hydrogen Sulfide in the Localization and Expression of p53 and Cell Death in the Nervous Tissue in Traumatic Brain Injury and Axotomy

doi: 10.3390/ijms242115708

Figure Lengend Snippet: Conceptual scheme of the role of H 2 S in the regulation of p53 levels in TBI and axotomy. Arrows with a sharp end—positive regulation; arrows with a blunt end—negative regulation.

Article Snippet: Sections are then incubated with rabbit primary anti-p53 antibody (1:100; PAA928Mu01, Cloud-Clone Corp, Wuhan, China) and mouse anti-neuN protein antibody (1:1000; FNab10266, FineTest, Wuhan, China) or astrocyte marker GFAP (1:1000; SAB4200571, Sigma Aldrich) for two days at 4 °C.

Techniques: